Reverse Complement DNA Tool

Paste a DNA sequence or FASTA record to get its reverse complement, reverse or complement. Everything runs in your browser and nothing is uploaded.

Input sequence

Convert to Reverse complement Reverse Complement

Result

How It Works

Reverse complement swaps each base for its pair (A↔T, G↔C) and reverses the order, giving the opposite strand read 5′→3′.
Case is preserved, so aTg becomes cAt. N stays N.
Only G A T C N are kept. Spaces, numbers, gaps, IUPAC ambiguity codes and RNA U are removed, and a warning is shown.
A FASTA header line (>title) is removed and shown in the results line. Convert one record at a time.
The result is one unwrapped line, ready to copy.

Output matches the Reverse Complement tool in the Sequence Manipulation Suite by Paul Stothard.